Development of Serological Ovine Field Test (SOFT) by Modified Agar-Gel Immunodiffusion
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چکیده
منابع مشابه
Serological differentiation of Brucella-vaccinated and -infected domesticated animals by the agar gel immunodiffusion test using Brucella polysaccharide in mongolia.
To investigate Brucella infection in cattle, sheep, goat, reindeer and yak in Mongolia, serological reactions of Brucella-infected and -vaccinated domestic animals were compared by the agar gel immunodiffusion (AGID) test with a polysaccharide (poly-B) of the B. Abortus strain S-19. The sensitivity and specificity were compared with conventional serological tests that are commonly used in Mongo...
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The value of various serological tests in the diagnosis of paracoccidioidomycosis was studied. Quantitative agar-gel immunodiffusion and indirect immunofluorescent tests were performed, and the results were compared with those of complement fixation and qualitative agar-gel procedures. The quantitative immunodiffusion procedure was found to serve as the simplest and safest quantitative test tha...
متن کاملComparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of Marek’s Disease Virus
A study was undertaken to identify Marek’s disease virus (MDV) antigen by PCR and AGID and to test the significance of PCR and AGID by McNemar’s test in detection of MDV antigen in outbreak in layer flocks. A total of twelve different MD outbreak flocks with varying flock size were selected in this study. Feather follicles were collected from 10 apparently healthy birds, 10 clinically affected ...
متن کاملAgar-gel immunodiffusion assay for pseudorabies virus antibody.
Soluble antigen prepared from pseudorabies virus-infected embryonic pig kidney cells was used to demonstrate the presence of pseudorabies virus antibodies in sera taken from naturally and experimentally infected swine. Antibody could be detected by agar-gel immunodiffusion as early as 14 days postexposure and was demonstrable at least 3 months after experimental infection (the longest period te...
متن کاملBovine leukemia virus infection in Taiwan: evaluation of the enzyme-linked immunosorbent assay and agar gel immunodiffusion test.
I evaluated an enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) test simultaneously for the detection of bovine leukemia virus (BLV) antibodies. Total 1,293 serum samples were tested for ELISA and AGID test and the results were compared. The results of ELISA and AGID agreed by 1,156 out of 1,293 (89.4%). All of AGID-positive 356 sera were positive by ELISA. However,...
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ژورنال
عنوان ژورنال: Journal of AOAC INTERNATIONAL
سال: 1989
ISSN: 0004-5756
DOI: 10.1093/jaoac/72.1.60